Journal: iScience

Article Title: CGK733 alleviates ovariectomy-induced bone loss through blocking RANKL-mediated Ca 2+ oscillations and NF-κB/MAPK signaling pathways

doi: 10.1016/j.isci.2023.107760

Figure Lengend Snippet:

Article Snippet: CGK733 (CG) , Med Chem Express , CAS: 905973-89-9.

Techniques: Recombinant, SYBR Green Assay, CCK-8 Assay, Reverse Transcription, ALP Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: BMB Reports

Article Title: Triglyceride induces DNA damage leading to monocyte death by activating caspase-2 and caspase-8

doi: 10.5483/BMBRep.2022-0201

Figure Lengend Snippet: TG induces DNA damage in THP-1 monocytes. (A) THP-1 monocytes were treated with or without TG for 24 h, and comet assays were performed to assess DNA damage. (B) THP-1 monocytes were incubated with the indicated concentration of TG for 24 h or with TG (1.0 mg/ml) for the indicated times. Phosphorylation of H2AX, Chk1, Chk2, ATR, and ATM was detected using western blot. THP-1 monocytes were treated with TG (1.0 mg/ml) in the absence or presence of the ATM/ATR inhibitor CGK733 for 24 h. (C) PARP cleavage was detected using western blot, and (D) viable cells were enumerated using trypan blue dye exclusion assay. The number of viable THP-1 monocytes without TG and inhibitor treatment was set as 100%. P-values were determined with Student’s t -test. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: The ATM/ATR kinase inhibitor CGK733 was purchased from Calbiochem (Darmstadt, Germany).

Techniques: Incubation, Concentration Assay, Phospho-proteomics, Western Blot, Exclusion Assay

Journal: International Journal of Biological Sciences

Article Title: Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress

doi: 10.7150/ijbs.17260

Figure Lengend Snippet: Binding of hMOF on Chromatin is Dependent on ATM Kinase Activity. (A) Chromatin fractionation analysis of hMOF binding on chromatin in HCT116 cells with or without 1 μM Wortmaninn treatment. The protein level of hMOF is normalized using Quantity One software. (B) Chromatin fractionation analysis of hMOF binding on chromatin in HCT116 cells treated with or without 1 μM ATM inhibitor (CGK733) treatment. The protein level of hMOF is normalized using Quantity One software. (C) Chromatin fractionation analysis of hMOF binding on chromatin in HCT116 cells treated with or without 1 μM DNA-PK inhibitor (NU7026). The protein level of hMOF is normalized using Quantity One software. (D) Detection of H4K16ac before and after treatment of Wortmaninn, CGK733, and NU7026, respectively. H4 and β-actin were detected as loading control. The protein level of H4K16ac is normalized using Quantity One software.

Article Snippet: In addition, the following reagents were used: Adriamycin (Sigma, D4035), Bleomycin (Sigma, B5507), Camptothecin (Sigma, C9911), Hydrogen peroxide solution 30% (w/w) (Sigma, H1009), Trichostatin A (Sigma, T8552), Wortmannin (Sigma, W1628), CGK733 (Sigma, C9867), and NU7026 (Sigma, N1537).

Techniques: Binding Assay, Activity Assay, Fractionation, Software